WB, IP, IHC
between 300 and 350
TRAP; Tumor necrosis factor type 2 receptor-associated protein 3; tumor necrosis factor type 2 receptor associated protein 3; TRAP3; RNF117; RING-type E3 ubiquitin transferase TRAF2; MGC:45012; E3 ubiquitin-protein ligase TRAF2; TNF receptor-associated fa
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Rabbit anti-TRAF2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)
2 - 8°C
Detection of human TRAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRAF2 antibody A303-46 1 A used for IP at 6 µg/mg lysate. TRAF2 was also immunoprecipitated by rabbit anti-TRAF2 antibody A303-460A, which recognizes an upstream epitope. For blotting immunoprecipitated TRAf2, A303-460A was used at 0.4 µg/ml,. Detection: Chemiluminescence with an exposure time 10 seconds.