CCT1, CCTa, CCT-alpha, D6S230E, tailless complex polypeptide 1, t-complex 1 protein, T-complex protein 1 subunit alpha, T-complex protein 1, alpha subunit, TCP-1-alpha
between 450 and 500
2 - 8°C
Detection of human and mouse TCP1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TCP1 antibody A303-444A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. TCP1 was also immunoprecipitated by rabbit anti-TCP1 antibodies A303-443A and A303-445A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).