ACLS, GCPS, GLI3 form of 190 kDa, GLI3 full-length protein, GLI3-190, GLI3FL, GLI-Kruppel family member GLI3, glioma-associated oncogene family zinc finger 3, oncogene GLI3, PAPA, PAP-A, PAPA1, PAPB, PHS, PPDIV, transcriptional activator GLI3, zinc finger
between 875 and 925
2 - 8°C
Detection of human GLI3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GLI3 antibody A303-417A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. GLI3 was also immunoprecipitated by rabbit anti-GLI3 antibody A303-418A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).