pre-mRNA-splicing factor SRP75, serine/arginine-rich splicing factor 4, SFRS4, splicing factor, arginine/serine-rich 4, SR splicing factor 4, SRP001LB, SRP75
Between 400 and 450
2 - 8°C
Detection of human SRp75 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SRp75 antibody A303-671A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. SRp75 was also immunoprecipitated by rabbit anti-SRp75 antibody A303-670A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human SRp75 by immunohistochemistry. Samples: FFPE serial sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-SRp75 (Cat. No. A303-670A Lot1, left image and Cat. No. A303-671A Lot1, right image) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).