www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

  We're currently experiencing shipment delays - find out more.
*

Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate and CF®594 Annexin V

Supplier:
Catalogue number:
BT30067
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£396.00 Estimated delivery Thu 3 Feb
Shipping is calculated in checkout

This kit provides two apoptosis markers, our novel NucView 488 Caspase-3 Substrate and Annexin V conjugated to red fluorescent CF594, for profiling apoptotic cells and studying the relation of two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation. The rate of apoptosis varies from cell to cell even within the same population. As a result, various apoptotic events or markers accompanying the apoptotic process also occur differently among cells. Thus, it is important to be able to detect these apoptotic events on an individual cell basis. Traditionally, caspase activity has been detected either using a membrane-impermeable fluorogenic enzyme substrate such as DEVD-R110, or a fluorescently-labeled inhibitor such as a FLICA reagent. In the former case, cell lysis is required, thus precluding the detection of caspase activity in live cells. In addition, such caspase assays measure only the average caspase activity of a highly heterogeneous cell population at a given time. In the latter case, although a FLICA reagent can enter live cells to detect caspase activity, only the initial fluorescent signal following the application of the reagent can truly reflect the enzyme activity or the state of the apoptotic cells because any detected signal after the initial "snapshot" will need to consider the potential interference of the inhibitor to the enzyme and the apoptotic cell itself.Unlike conventional caspase assays, NucView 488 Caspase-3 substrate detects caspase-3 activity within individual whole cells in a non-interfering manner. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView 488 caspase-3 substrate is bi-functional, allowing detection of caspase-3 activity and visualization of apoptotic nuclear morphology.The kit contains reagents sufficient for 50 flow cytometry assays (200 uL assay volume). The number of fluorescence microscopy assays that can be performed with the kit may vary based on the size of culture vessel and staining volume used.See our full selection of NucView substrates and kits.NucView enzyme substrate technology is covered by U.S. patents.

Product Type:

Biochemical and cellular assays

Storage Temp:

2°C to 8°C

UNSPSC Code:

41116133

BT30067: Dual Apoptosis Assay with NucVi

BT30067: Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate and CF®594 Annexin V (50 assays)

BT30067: Dual Apoptosis Assay with NucVi

BT30067: Dual Apoptosis Assay with NucView® 488 Caspase-3 Substrate and CF®594 Annexin V (50 assays)

Details Cat number & supplier Size Price
ATP-Glo™ Bioluminometric Cell Viability Assay BT30020-T · Biotium
BT30020-T
Biotium
1 kit £38.00
1 kit
view
Annexin V, R-PE Conjugate BT29045-100uL · Biotium
BT29045-100uL
Biotium
100 ul £109.00
100 ul
view
Annexin V, R-PE Conjugate BT29045-500uL · Biotium
BT29045-500uL
Biotium
500 ul £289.00
500 ul
view
Annexin V, CF®405M Conjugate BT29009 · Biotium
BT29009
Biotium
0.5 ml £282.00
0.5 ml
view
Ac-DEVD-CHO, Caspase-3 Inhibitor BT10404-1 · Biotium
BT10404-1
Biotium
1 mg £78.00
1 mg
view
Ac-DEVD-CHO, Caspase-3 Inhibitor BT10404 · Biotium
BT10404
Biotium
5 mg £267.00
5 mg
view
Apoptotic, Necrotic and Healthy Cells Quantitation Kit Plus BT30066 · Biotium
BT30066
Biotium
1 kit £199.00
1 kit
view