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Apoptosis and Necrosis Quantitation Kit Plus

Supplier:
Catalogue number:
BT30065
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£199.00 Estimated delivery Thu 27 Jan
Shipping is calculated in checkout

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane for phagocytic cell recognition. The human anticoagulant Annexin V is a 35 kDa, Ca2 -dependent phospholipid-binding protein with high affinity for PS. Annexin V labeled with CF488A (Abs/Em: 490/515 nm) stains apoptotic cells green by binding to PS exposed on the cell surface. CF488A is spectrally similar to fluorescein (FITC), with much brighter and more photostable fluorescence. Necrosis normally results from severe cellular insult. Both internal organelle and plasma membrane integrity are lost, resulting in the release of cellular contents into the surrounding environment. Ethidium homodimer III (EthD-III) is a highly positively charged nucleic acid probe, which is impermeant to live cells, but stains cells with compromised membrane integrity with red fluorescence (Abs/Em (with DNA): 528/617 nm). EthD-III is a superior alternative to propidium iodide (PI) or ethidium homodimer I because of its significantly higher affinity for DNA and higher fluorescence quantum yield. The Apoptosis Necrosis Quantitation Kit Plus provides a convenient assay for quantifying apoptotic (green) and necrotic (red) cells within the same cell population by flow cytometry or fluorescence microscopy. Kit Components: CF488A-Annexin V Ethidium homodimer III 5X binding buffer Features: Simple: Simultaneous detection of both apoptotic and necrotic cells in a single assay Fast: A single 15-30 min staining step Sensitive: CF488A-Annexin V is brighter and more photostable than FITC-Annexin V, while EthD-III is a superior alternative to both PI and EthD-I for staining necrotic cells.

Product Type:

Biochemical and cellular assays

Storage Temp:

2°C to 8°C

UNSPSC Code:

41116133

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