Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (TA302197) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (TA302197)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) (Cat.#TA302197), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.