NANOG Rabbit Polyclonal Antibody

Name: NANOG Rabbit Polyclonal Antibody
Brand: OriGene
SKU: CBSKU_00140896
Price: 698 GBP
Availability: InStock

Catalogue number:

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TA302153

Supplier:

OriGene

Size:

400 µl _$$_

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£698.00

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Applications:

FC, IF, IHC, WB

Antibody Host:

Rabbit

Species Reactivity:

Human (Predicted: Monkey)

Antibody Isotype:

Antibody Type:

Polyclonal

Immunogen:

This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region o

Alternative Names:

homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox

Data sheet: View or download
MSDS: View or download

Product Description:

Rabbit Polyclonal Antibody against NANOG (N-term)

Storage Temperature:

Store at -20°C as received.

Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term) (Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IF image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

IF image of Hela cell stained with NANOG Antibody (N-term) (Cat#TA302153).Hela cells were incubated with NANOG primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). NANOG immunoreactivity is localized to Nucleus significantly.

NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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