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NANOG Rabbit Polyclonal Antibody

Supplier:
Catalogue number:
TA302153
Size:
100 µg
Product is available in:
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Rabbit Polyclonal Antibody against NANOG (N-term)

Product Type:

Antibodies

UNSPSC Code:

12352203

Western blot analysis of anti-NANOG Anti

Western blot analysis of anti-NANOG Antibody (N-term) (Cat.#TA302153) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat.#TA302153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded hum

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term) (Cat.#TA302153), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IF image of SY5Y cells stained with TA30

IF image of SY5Y cells stained with TA302153 NANOG (N-term) antibody. SY5Y cells were incubated with TA302153 NANOG (N-term) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin. Nuclei were counterstained with Hoechst 33342 (blue). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

IF image of Hela cell stained with NANOG

IF image of Hela cell stained with NANOG Antibody (N-term) (Cat#TA302153).Hela cells were incubated with NANOG primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). NANOG immunoreactivity is localized to Nucleus significantly.

NANOG Antibody (N-term) (Cat. #TA302153)

NANOG Antibody (N-term) (Cat. #TA302153) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.