Conducted in state-of-the-art lipid analysis laboratories, Cayman's untargeted lipidomics profiling enables the identification of profile changes across all major lipid classes, or within a single lipid class, including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids and sterol lipids.
Untargeted lipidomics service process
- Lipids are extracted from samples using liquid-liquid methods
- Lipids are chromatographically separated, using HPLC or UPLC to detect individual molecular species & identify changes between samples
- High-resolution mass spectrometry (HRMS) & tandem mass spectrometry (MS/MS) are used to detect & identify lipid species
Data-processing software and publicly available databases facilitate the identification and relative quantitation of candidate lipid species. When molecular identification is provided based on the LC-MS/MS data available, Cayman adheres to the recommendations issued by the Lipid Maps® consortium and the Lipidomics Standards Initiative.
Data is reported as tables of mass-to-charge ratios (m/z), with ion intensity peak areas normalised to internal standards, which can also be visualised using powerful plotting and graphing tools. Statistical analysis can also be applied to identify significant changes within complex lipid profiles.
2D feature plot (m/z vs retention time) generated using Lipostar lipidomics software package representing identified molecular species, corresponding to different lipid classes, in human plasma.
To find out more or discuss your project with us, please contact our lipidomics service specialists.
Lipid Maps is a registered trademark of The Regents of the University of California