The plexWell normalising library prep platform utilises a transposase to selectively tag DNA samples in a unique sequential manner, providing more control than other transposon methods with less bias and input DNA variations.
Find out more about the plexWell DNA library prep range.
How does the plexWell technology work?
Samples are sequentially tagged with Illumina P7 and P5 primers resulting in normalised libraries with unique barcode combinations, providing sequencer-ready libraries.
Unfragmented DNA is tagged with sample-specific i7-barcoded adapters followed by pooled barcoding. Pooled sample-barcoded DNA is tagged with pool-specific i5-barcoded adapters. The final step is library amplification with universal primers and SPRI purification. The pooled library is ready to be loaded in an Illlumina™ sequencer, producing normalised sequencing reads.
To discuss your requirements or to find out more about the plexWell range, please contact our seqWell specialist.
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