Whole genome bisulphite sequencing library preparation

The Zymo-Seq WGBS Library Kit from Zymo Research enables whole genome bisulphite sequencing (WGBS) library preparation in less than four hours, in a single tube. Featuring a streamlined workflow, the Zymo-Seq WGBS kit incorporates tagmentation technology to reduce library preparation bias, resulting in accurate methylation calling without the need for tedious ligation-based library preparation methods. 

As a gold standard for DNA methylation studies, the Zymo-Seq WGBS Library Kit provides base-level methylation quantification for all cytosines and enables reproducible genomic coverage from any species. The kit accomodates genomic DNA input from 10-100ng, making it compatible with a wide-range of DNA methylation analysis applications.

Benefits of using the Zymo-Seq WGBS Library kit

• Streamlined workflow, eliminates fragmentation, enzymatic and clean-up steps
• Ideal for high throughput applications
• Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples) 
• Includes 12 Unique Dual Index Primer Sets
• Libraries are compatible with Illumina® sequencing platforms

Enzymatic reactions are consolidated in a single tube

Intact genomic DNA is first bisulphite converted, which then undergoes second strand synthesis (1). Tagmentation reagents are added directly to the reaction to tag adapters onto the double-stranded DNA (2). Indexing primers and PCR mixture are added to amplify the WGBS library (3). Purified libraries are ready for sequencing on Illumina instruments.

Applications of the Zymo-Seq WGBS library prep kit

• Whole-genome methylation calling of CG, CHG, and CHH sites

Material available for download

Zymo-Seq WGBS Library Kit datasheet
Zymo-Seq WGBS Library Kit protocol

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