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High-throughput SARS-CoV-2 RNA extraction kits

To meet the high demand for viral RNA extraction during the COVID-19 pandemic, Zymo Research have streamlined their manufacturing to produce large numbers of the Quick-DNA/RNA Viral MagBead Kit (R2141). With a simple protocol and compatible with multiple samples types, high quality SARS-CoV-2 RNA can be extracted in just 1-2 hours (depending on the automation platform) using this kit. These kits can be used to process samples manually on the bench, or will work on any open automation platform. For automation support, please contact our technical specialists.

Virus inactivation
The kit contains DNA/RNA Shield reagent.  DNA/RNA Shield has demonstrated to inactivate the MERS-coronavirus and a wide range of other pathogens.
Citations demonstrating the inactivation of viruses using DNA/RNA Shield can be found here.
More detailed information about DNA/RNA Shield can be found here.

Worldwide kit use
The Quick-DNA/RNA Viral MagBead kits are in use in numerous NHS and private testing labs in the UK, as well as globally in public and private COVID testing labs. These kits also form part of the EUA granted to Zymo Research as part of their COVID testing workflow.

Benefits of using the Quick-DNA/RNA Viral MagBead Kit
• Short lead times for delivery
• Fast - 96 samples extracted in 1-2hrs depending on platform used
• Automation compatible (scripts and automation support available)
• Manual protocol available
• Compatible with swabs, sputum, saliva, samples collected in DNA/RNA Shield, samples collected in universal transport medium (UTM/VTM) or similar and more

High quality SARS-CoV-2 RNA extraction

High quality SARS-CoV-2 RNA extraction

RT-qPCR analysis of total RNA isolated from sputum using the Quick-DNA/RNA Viral MagBead Kit. Positive control sample was spiked with a non-infectious in vitro COVID-19 synthetic RNA. Negative control sample was not spiked with in vitro RNA. Samples were tested using primers targeting the human RNase P gene (“RNase P”, amplification control) or primers 2019 nCoV_N3 (CV-3).

Catalogue number Size Input Binding capacity Elution volume Equipment required
R2141 4 x 96 preps ≤ 400 µl plasma, serum, urine, cell culture media, blood, saliva, cellular suspension, swab, fecal sample or ≤ 5 mg biopsy sample. 10 µg DNA/RNA per 20 µl MagBinding Beads ≥ 50 µl Manual: Magnetic stand or separator

Automated:
Magnetic stand or separator
Liquid handler or robotic sample processor

Associated products: ZR-96 MagStand (P1005), Collection Plate (C2002; capacity 1.2 ml/well), 96-Well Block (P1001; capacity 2 ml/well), Elution Plate (C2003; capacity 0.35 ml/well) and Cover Foil (C2007)

Need automation support?
For automation support, contact our technical specialists

Material available for download
Quick-DNA/RNA Viral MagBead kit protocol
Quick-DNA/RNA Viral MagBead kit automation reference guide
Quick-DNA/RNA Viral MagBead kit nose and throat swab manual protocol

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Quick-DNA/RNA Viral MagBead R2141 · Zymo Research
R2141
Zymo Research
4 x 96 preparations £1023.00
4 x 96 preparations
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