T7 cloning and expression systems: Expresso

The Expresso® T7 cloning and protein expression systems from Lucigen are designed for fast, easy and efficient directional cloning and expression of PCR-amplified genes. The systems are complete with pre-processed pETite™ N- or C-His cloning vectors, and two competent cell lines, supplied in single transformation vials. The Expresso T7 cloning and protein expression systems uses Expressioneering Technology™, an enzyme-free recombinational cloning strategy to seamlessly integrate the gene with the vector

Benefits of using the Expresso cloning and protein expression systems
• Enzymo-free cloning in seconds with cloning-ready vector and cells
• High efficiency: >90% recombinants
• Tightly-controlled expression of N- or C- terminal 6xHis tagged protein
• SUMO tag vector available for low solubility protein expression

Comparison of Expressioneering technology and traditional cloning steps

High cloning efficiency with Expresso T7 system

Pre-processed pETite C-His vector was mixed with 1 µl of unpurified PCR gene product and transformed into HI-Control 10G Chemically Competent Cells. Colony PCR was performed on 18 randomly chosen colonies; 17 of 18 contained insert of the correct size.

Expresso T7 system
Contains pre-processed pETite® N-His and/or pETite C-His Vector DNA, two competent cell lines optimised for cloning and expression and N-His and/or C-His Posi pre-processed pETite™ N- or C-His cloning vectors.

Expresso T7 SUMO system
Contains pre-processed pETite® N-His SUMO Vector DNA, two competent cell lines optimised for cloning and expression, SUMO Positive Control C Insert DNA, transformation positive control pUC DNA, SUMO Express Protease, SUMO Cleavage Control Protein and forward and reverse PCR primers to confirm clones.

Material available for download
Expresso T7 cloning and expression system manual
Expresso T7 SUMO cloning and expression system manual
Poster: Expresso cloning and expression systems
Nature application note: Expresso cloning and expression sysems: Expressioneering technology streamlines recombinant protein expression