The packaging plasmid mix pPACK™ from System Biosciences (SBI) produces all the structural and replication proteins needed to generate VSV-G-pseudotyped lentiviral particles containing an expression construct (constructed using SBI RNAi vectors, pGreenFire™ or pGreenZeo™ reporters, microRNA or cDNA vectors). Enabling cells to replicate expression constructs and package them into pseudoviral particles, the pPACK packaging plasmid mix efficiently packages the expression construct into highly transducible VSV-G-pseudotyped lentiviral particles.
Benefits of using the pPACK lentivector packaging systems
• High titre, transduction-ready pseudoviral particle generation
• Efficient delivery of constructs to a wide range of mammalian cells
• Stable lentivector construct expression
• Quantify pseudoviral titres and transduction efficiency using copGFP control
• FIV- and HIV-based lentivector packaging kits available
High titre lentivirus packaging systems
SBI’s pPACKH1 and pPACKF1 Lentivector Packaging Systems possess broad cellular tropism
Material available for download
Guide to Lentivector Packaging and Transduction of Target Cells
Safety guidelines with lentiviral delivery systems
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| pPACKF1 FIV Lentivector Packaging Kit LV100A-1 · System Biosciences | LV100A-1 System Biosciences |
10 transfections |
£578.00
10 transfections
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| pPACKH1 HIV Lentivector Packaging Kit LV500A-1 · System Biosciences | LV500A-1 System Biosciences |
10 transfections |
£552.00
10 transfections
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| pPACKH1-XL HIV Lentivector Packaging Kit LV510A-1 · System Biosciences | LV510A-1 System Biosciences |
25 transfections |
£1216.00
25 transfections
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| 293TN Producer Cell Line LV900A-1 · System Biosciences | LV900A-1 System Biosciences |
2 million cells |
£362.00
2 million cells
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