Gene editing quantification kits

The dGH in-Site CAR-T probe kits from KromaTiD enable quantitative measurement of rearrangements at the edit site, providing unbiased detection of CAR insertions and structural variants as small as 2 Kb. These kits are ideal for researchers looking to understand the genomic structural outcomes of their cellular engineering processes.

What is directional genomic hybridisation?
Directional genomic hybridisation (dGH) is the only genomics approach for single cell whole genome analysis capable of detecting DNA sequence, location and orientation in a single test. This powerful technique provides single cell visualisation of structural variations commonly caused by CAR T-cell engineering processes such as CRISPR-Cas9 editing.

Benefits of using the dGH in-Site CAR-T Probe Kit 
•    Direct visualisation of DNA sequence, location and orientation of CAR-T genomic loci
•    Track cellular engineering impact on TRAC and B2M loci
•    Ultra-high resolution as low as 2 Kb
•    High signal-to-noise ratio
•    Lower limit of detection than FISH/BAC FISH
•    Identifies previously undetectable structural rearrangements

dGH in-Site CAR-T probe kits allow for an easy workflow

Single stranded probes are designed to target only the parental strand

dGH workflow results in high quality images during analysis

Applications
•    Genotoxicity studies
•    CRISPR/Cas9 gene therapy
•    Gene editing optimisation
•    Quality control in editing trials/experiments
•    Identifying previously undetected chromosomal mutations
•    Oncogenic mutations identification
•    Structural damage in cellular reagent delivery