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CUT&Tag-IT assay kit

The CUT&Tag-IT™ assay kit from Active Motif enables rapid and robust genome-wide analysis of histone marks and transcription factor binding using in as few as 5,000 cells, making it ideal for researchers with limited samples. With no need for cell fixation and no artifacts caused from formaldehyde crosslinking, this CUT&Tag method produces high-quality, sequencing-ready libraries without the laborious and costly steps of ChIP-Seq.

Benefits of using the CUT&Tag-IT assay kit
• Compatible with as few as 5,000 cells
• Genome-wide analysis of histone marks and transcription factor binding
• Sequencing-ready libraries produced
• Low background signal for lower sequencing depth
• No artifacts caused by formaldehyde crosslinking
• Provided as a complete kit with optimised protocol

Please note that for researchers interested in understanding genome-wide transcription factor recruitment (endogenous or tagged), ChIP-seq is still recommended. Explore the range of ChIP-seq assay kits from Active Motif.

How the CUT&Tag-IT assay works

How CUT&Tag-IT works

CUT&Tag comparison against CUT&RUN and ChIP-Seq

  CUT&Tag CUT&RUN ChIP-Seq
Performed under native conditions? Yes Yes No
Chromatin fragmentation method Tn5-based tagmentation MNase digestion Sonication
Cell number requirements 5000-500,000 cells 500,000 cells 1-10 million cells
Sequencing depth required* 2 million reads** 8 million reads 20-50 million reads
Integrated library preparation Yes, uses tagmentation No, separate library prep required No, separate library prep required
Compatible targets Primarily histone modificatons, some transcription facors and co-factors Wide range of histone modifications, transcription factors and co-factors Wide range of histone modifications, transcription factors and co-factors
Workflow length 1-2 days 1-2 days 2-3 days

* Kaya-Okur et al. Nature Communications (2019) 10:1930
** For less abundant targets of interest, 8 to 10 million reads are recommended

Material available for download
CUT&Tag-IT Assay Kit manual

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CUT&Tag-ITâ„¢ Assay Kit 53160 · Active Motif
53160
Active Motif
16 reactions £1161.00
16 reactions
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