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- CUT&Tag-IT Assay Kit manual
The CUT&Tag-IT™ assay kit from Active Motif enables rapid and robust genome-wide analysis of histone marks and transcription factor binding using in as few as 5,000 cells, making it ideal for researchers with limited samples. With no need for cell fixation and no artifacts caused from formaldehyde crosslinking, this CUT&Tag method produces high-quality, sequencing-ready libraries without the laborious and costly steps of ChIP-Seq.
Benefits of using the CUT&Tag-IT assay kit
• Compatible with as few as 5,000 cells
• Genome-wide analysis of histone marks and transcription factor binding
• Sequencing-ready libraries produced
• Low background signal for lower sequencing depth
• No artifacts caused by formaldehyde crosslinking
• Provided as a complete kit with optimised protocol
Please note that for researchers interested in understanding genome-wide transcription factor recruitment (endogenous or tagged), ChIP-seq is still recommended. Explore the range of ChIP-seq assay kits from Active Motif.
How the CUT&Tag-IT assay works
CUT&Tag comparison against CUT&RUN and ChIP-Seq
| CUT&Tag | CUT&RUN | ChIP-Seq | |
|---|---|---|---|
| Performed under native conditions? | Yes | Yes | No |
| Chromatin fragmentation method | Tn5-based tagmentation | MNase digestion | Sonication |
| Cell number requirements | 5000-500,000 cells | 500,000 cells | 1-10 million cells |
| Sequencing depth required* | 2 million reads** | 8 million reads | 20-50 million reads |
| Integrated library preparation | Yes, uses tagmentation | No, separate library prep required | No, separate library prep required |
| Compatible targets | Primarily histone modificatons, some transcription facors and co-factors | Wide range of histone modifications, transcription factors and co-factors | Wide range of histone modifications, transcription factors and co-factors |
| Workflow length | 1-2 days | 1-2 days | 2-3 days |
* Kaya-Okur et al. Nature Communications (2019) 10:1930
** For less abundant targets of interest, 8 to 10 million reads are recommended
Material available for download
CUT&Tag-IT Assay Kit manual
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| CUT&Tag-IT™ Assay Kit, Anti-Rabbit 53160 · Active Motif | 53160 Active Motif |
16 reactions |
£1445.00
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