Nucleic acid labelling kits: Label IT

The Label IT® technology from Mirus Bio is a non-enzymatic, chemical labelling method for direct covalent attachment of either fluorescent or non-fluorescent labels to any nucleic acid. Using a simple, one-step chemical reaction, these kits are optimised for specialised applications including nucleic acid visualisation both in vitro and in vivo. 

How the Label IT Nucleic Acid Labelling Kits work

Label IT Nucleic Acid Labelling Kits - Label IT chemical labelling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® reagent to any reactive heteroatom within the nucleic acids. 

Benefits of using the Label IT kits
• Label any DNA or RNA template
• Single-step chemical method
• Adjustable labelling density
• Covalent mechanism does not impact hybridisation performance
• Label and deliver plasmid DNA for intracellular tracking experiments
• Tracking of functional fluorescently labelled siRNA 

Successful delivery and functional knockdown using labelled siRNA

(A) HeLa cells in 12-well plates were transfected at 70% confluence with TransIT-TKO® Transfection Reagent (3 μl/well) and Label IT siRNA Tracker Fluorescein-labelled siRNA duplexes (GREEN, 50 nM final concentration in the well). The cells were incubated 24 hours post-transfection then fixed and counterstained with TO-PRO®-3 (nuclei, BLUE) and Alexa Fluor® 546 Phalloidin (actin, RED). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope. (B) TransIT-TKO Transfection Reagent was used to transfect anti-firefly luciferase siRNA into CHO-Luc cells stably expressing firefly luciferase. The siRNA was either unlabeled or labeled with Label IT siRNA Tracker Cy®3, Cy®5, Fluorescein, or CX-Rhodamine Reagents. Bars indicate the percent firefly luciferase expression 24 hours after delivery of 5 nM anti-firefly luciferase siRNA.

Material available for download
Label IT Nucleic Acid Labelling Kits protocol
Label IT Nucleic Acid Labelling Kits quick reference protocol
Label IT Nucleic Acid Labelling Kits SDS
Label IT Amine Full Labelling protocol
Label IT siRNA Tracker™ Kit Full Labelling protocol
Label IT Tracker Kit Full Labelling protocol
Technical report: High efficiency siRNA delivery in vitro
Intracellular localisation and expression of labelled plasmid DNA using Label IT Tracker Nucleic Acid Labelling Kits
Nucleic acid labelling and delivery tools for neurobiology

Contact a specialist
To discuss your requirements, please contact our Mirus specialists.

TO-PRO and Alexa Fluor are registered trademarks of Thermo Fisher Scientific. Cy is a registered trademark of GE Healthcare UK Limited.