The promoter cloning and expression lentivectors from System Biosciences (SBI) are effective in delivery and stable expression of any cDNA in a wide range of mammalian cells. With a wide selection of promoter options available including CMV (Cytomegalovirus), MSCV (Murine Stem Cell Virus), UbC (Ubiquitin C), PGK (Phosphoglycerate Kinase) and EF1 (Elongation Factor 1α), deliver your cDNA construct of interest to both dividing and non-dividing cells.
Benefits of using these lentivectors
• Multiple promoter options
• Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
• Single promoter, dual promoter and bidirectional promoter formats
• High expression in most hematopoietic, embryonic and stem cells via the MSCV CpG-deficient promoter
| Promotor | Expression level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080 etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
Effective transduction with SBI’s bidirectional promoter lentivectors
HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.
Material available for download
pCDH cDNA cloning and expression lentivectors manual
pCDF cDNA cloning and expression lentivectors manual
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| pCDH-EF1-MCS-IRES-Neo cDNA Cloning and Expression Vector CD533A-2 · System Biosciences | CD533A-2 System Biosciences |
10 µg |
£638.00
10 µg
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| pCDH-EF1-MCS-BGH-PGK-GFP-T2A-Puro cDNA Cloning and Expression Vector CD550A-1 · System Biosciences | CD550A-1 System Biosciences |
10 µg |
£611.00
10 µg
view
|
| pCDH-UbC-MCS-EF1-Hygro cDNA Cloning and Expression Vector CD615B-1 · System Biosciences | CD615B-1 System Biosciences |
10 µg |
£618.00
10 µg
view
|
| pCDH-UbC-MCS-IRES-GFP cDNA Cloning and Expression Vector CD630A-1 · System Biosciences | CD630A-1 System Biosciences |
10 µg |
£638.00
10 µg
view
|
| pCDH-MSCV-MCS-EF1-Puro cDNA Cloning and Expression Vector CD710B-1 · System Biosciences | CD710B-1 System Biosciences |
10 µg |
£618.00
10 µg
view
|
| pCDH-MSCV-MCS-EF1-GFP cDNA Cloning and Expression Vector CD711B-1 · System Biosciences | CD711B-1 System Biosciences |
10 µg |
£618.00
10 µg
view
|
| pCDH-MSCV-MCS-EF1-GFP+Puro cDNA Cloning and Expression Vector CD713B-1 · System Biosciences | CD713B-1 System Biosciences |
10 µg |
£618.00
10 µg
view
|
| pCDH-MSCV-MCS-IRES-GFP cDNA Cloning and Expression Vector CD731B-1 · System Biosciences | CD731B-1 System Biosciences |
10 µg |
£638.00
10 µg
view
|
| pPS-UBC-GFP-RFP (plasmid) LV606PA-1 · System Biosciences | LV606PA-1 System Biosciences |
10 µg |
£642.00
10 µg
view
|
| pPS-MSCV-GFP-RFP (plasmid) LV605PA-1 · System Biosciences | LV605PA-1 System Biosciences |
10 µg |
£642.00
10 µg
view
|
| pPS-PGK-GFP-RFP (plasmid) LV604PA-1 · System Biosciences | LV604PA-1 System Biosciences |
10 µg |
£642.00
10 µg
view
|
| pPS-EF1-GFP-RFP (plasmid) LV603PA-1 · System Biosciences | LV603PA-1 System Biosciences |
10 µg |
£642.00
10 µg
view
|
| pCDH-CMV-MCS-EF1-Puro cDNA Cloning and Expression Vector CD510B-1 · System Biosciences | CD510B-1 System Biosciences |
10 µg |
£618.00
10 µg
view
|
