BioFX and Surmodics provide in vitro diagnostic immunoassay technologies. Their wide range of colourimetric and chemiluminescent BioFX® substrates for microwell and membrane applications are available in a variety of sensitivities for ELISA, Western Blot, ELISpot and immunohistochemistry applications.
Excellent signal generation
An IL-6 immunoassay (R&D Systems) was conducted using TMBHD, TMBW, and various other high-sensitivity TMB substrates to compare their limits of detection and sensitivity. A standard curve was generated, ranging from 100 fg/well to 6.25 fg/well of IL-6. The signal was plotted as the optical density at 450 nm (OD450) of each well, minus the background (zero standard) value. The detection limit was determined using a Student’s T test (p < 0.05) at the lowest analyte concentration tested.
Cambridge Bioscience is pleased to provide the BioFX® range of products from Surmodics in the UK and Ireland.
