The octapeptide angiotensin II exerts a wide range of effects on the cardiovascular system. It is also implicated in the regulation of cell proliferation, fibrosis and apoptosis. Angiotensin II is formed by cleavage of angiotensin I by angiotensin-converting enzyme (ACE) or chymases. Human heart chymase, a chymotrypsin-like serine proteinase, hydrolyses the Phe8-His9 bond to yield the octapeptide hormone angiotensin II and His-Leu.
In PBS pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin