Caffeine (1,3,7-trimethylxanthine) is a methylxanthine alkaloid which acts as a stimulant for central nervous system that can be used to increase blood pressure and reduce fatigue. Caffeine is naturally present in leaves, seeds, nuts and a number of plants. It is also known to have anti-inflammatory effect by inhibiting adenosine monophosphate phosphodiesterase. Nowadays, many common beverages including coffee, tea, sodas and energy drinks contain different levels of caffeine to relieve drowsiness and improve performance. At normal dose, caffeine generally improves reaction time, wakefulness, concentration and motor coordination. However, caffeine overdose in the body can lead to nervousness, bone loss, headache, anxiety, insomnia and even death. BioVision’s Caffeine ELISA Kit is a competitive-based ELISA that can quantify as low as 0.3 ng/ml caffeine in urine, saliva and serum samples within 90 minutes.