Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

Name: Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads
Brand: OriGene
SKU: CBSKU_100708861
Price: 924 GBP
Availability: InStock

Catalogue number:

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TA150039

Supplier:

OriGene

Size:

1 ml _$$_

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£924.00

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Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic Beads

Data sheet: View or download
MSDS: View or download

Storage Temperature:

Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary.

Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.

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