SensoLyte520 Meprin α Assay

Meprin α is a zinc endopeptidase of the astacin family and the metzincin superfamily. Meprin α can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin , SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin α is associated with cancer proliferation, cancer migration and invasion.
The FRET substrate included in SensoLyte® 520 Meprin α Activity Assay Kit was designed to detect Meprin α activity and reduce the cross reactivity with Meprin β, ADAM10, β-secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin α cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin α.