Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Store at 4°C for short term storage. Long time storage is recommended at -20°C.
Immunofluorescence staining of U-251 cells using the anti-SOX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunofluorescence staining of mouse embryo E11 shows nuclear immunoreactivity in the developing brain, eye and trigeminal ganglion.
Immunofluorescence staining of mouse embryo E11 shows nuclear immunoreactivity in the developing neural tube.
Immunohistochemical staining of human cervix shows nuclear immunoreactivity in basal layer cells of squamous epithelium.
Immunohistochemical staining of human placenta shows no positivity as expected (negative control).
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western blot analysis in human cell line NTERA-2.