The Staphylococcus aureus transpeptidase Sortase A (SrtA) anchors virulence and colonisation-associated surface proteins to the cell wall. SrtA selectively recognises a C-terminal LPXTG motif. SrtA readily reacts with its native substrate Abz-LPETG-Dap(DNP)-NH2, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this FRET substrate can be monitored at Ex/Em=320 nm/420 nm.
Proteins & Peptides
Peak Area by HPLC ≥95%
Bentley, M. et al. J. Biol. Chem. 282, 6571 (2007).