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Caspase 3 (Apopain) Substrate 1f, fluorogenic - ANA25273-5 - 5 mg - AnaSpec - proteins & peptides

Caspase 3 (Apopain) Substrate 1f, fluorogenic
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Size: 5 mg
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Caspase 3 (Apopain) Substrate 1f, fluorogenic

AFC (7-amino-4-trifluoromethylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AFC peptides by caspases generate strongly fluorescent AFC that is monitored fluorimetrically at 500-510 nm with excitation of 370-390 nm.

Product Type:
Proteins & Peptides
Storage Temp:
-20°C desiccated and protected from light
Compound Purity:
Minimum Purity: 95%
Molecular Weight:
Gurtu V, et al. (1997). Fluorometric and colorimetric detection of caspase activity associated with apoptosis. Anal Biochem 251, 98-102; Martins LM, et al. (1997). Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis. J Biol Chem 272, 7421-30; Deveraux QL, et al. (1997). X-linked IAP is a direct inhibitor of cell-death proteases. Nature 388, 300-4; Xiang J, et al. (1996). BAX-induced cell death may not require interleukin 1 ?-converting enzyme-like proteases. Proc Natl Acad Sci USA 93, 14559-63.
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