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  3. PLK1 Mouse Monoclonal Antibody [Clone ID: OTI6C6]
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PLK1 Mouse Monoclonal Antibody [Clone ID: OTI6C6]

Catalogue number:
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TA500401
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
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£533.00
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Applications:

IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Mouse; Rat

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.

Alternative Names:

PLK; STPK13

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Product Description:

PLK1 mouse monoclonal antibody, clone OTI6C6 (formerly 6C6)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1. (1:. Positive lysates LY417592 (100ug) and LC417592 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PLK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500401)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500401)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500401)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500401)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500401)
Immunoprecipitation (IP) of PLK1 by usin
Immunoprecipitation (IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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