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PLK1 Mouse Monoclonal Antibody (Clone ID: OTI8C12)

Supplier:
Catalogue number:
TA500393
Size:
100 µl
Product is available in:
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Anti-PLK1 mouse monoclonal antibody, clone OTI8C12 (formerly 8C12)

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1. (1:. Positive lysates LY417592 (100ug) and LC417592 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PLK1 monoclonal antibody (1:500).

Anti-PLK1 mouse monoclonal antibody (TA5

Anti-PLK1 mouse monoclonal antibody (TA500393) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1 (RC201795) (1:100).

Immunoprecipitation (IP) of PLK1 by usin

Immunoprecipitation (IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

HEK293T cells transfected with either RC

HEK293T cells transfected with either RC201795 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody (TA500393), and then analyzed by flow cytometry (1:100).