HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and AK1-Knockout HeLa cells (KO, Cat# LC810761) were separated by SDS-PAGE and immunoblotted with anti-AK1 monoclonal antibody TA500323 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.