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AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI18C7 (formerly 18C7)

Supplier:
Catalogue number:
TA500323
Size:
100 µl
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AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI18C7 (formerly 18C7) - 100 µl

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.

Equivalent amounts of cell lysates (10 u

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and AK1-Knockout HeLa cells (KO, Cat# LC810761) were separated by SDS-PAGE and immunoblotted with anti-AK1 monoclonal antibody TA500323 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Anti-AK1 mouse monoclonal antibody (TA50

Anti-AK1 mouse monoclonal antibody (TA500323) immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY AK1 (RC215130).

HEK293T cells transfected with either RC

HEK293T cells transfected with either RC215130 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK1 antibody (TA500323), and then analyzed by flow cytometry.