The Stool DNA Purification Kit is designed for the rapid and reliable purification of high-quality DNA from stool or fecal samples. Up to 0.25 gm of stool samples can be processed in less than an hour. The kit protocol does not need organic extraction and multiple samples can be processed in parallel. The kit combines the reversible nucleic acid-binding properties of spin columns with a propriety buffer system to eliminate PCR inhibiting compounds such as humic acid from stool samples. In this kit, stool samples are homogenised and then treated in a specially formulated buffer that contains a detergent. Humic acid, proteins, polysaccharides and other contaminants are subsequently precipitated after a heat-freeze step. DNA is further purified using a spin-column. A rapid wash step removes trace contaminants and pure DNA is eluted in sterile water or low ionic strength Elution Buffer. Purified DNA can be used in downstream applications such as PCR, restriction digestion, and hybridisation techniques.
Purification and cleanup