RRBS library preparation

The Zymo-Seq RRBS library kit from Zymo Research offers an all-in-one solution for Reduced Representation Bisulphite Sequencing (RRBS) library preparation. Featuring a simplified workflow with as little as 2 hours hands-on time, the Zymo-Seq kit enables RRBS library preparation from as little as 10 ng genomic DNA, for unbiased methylation calling and reproducible CpG coverage.

RRBS is a targeted DNA methylation analysis method that uses enzymatic digestion followed by library preparation and NGS to analyse the methylation status of regions of the genome such as CpG islands, promotors, gene bodies and enhancers. This more targeted alternative to WGBS enables researchers to analyse genome-wide DNA methylation information in a cost-effective manner for large-scale screening studies.

Benefits of using the Zymo-Seq RRBS Library Kit

• All-in-one RRBS library prep solution
• Libraries cover a significant percentage of CpG islands, promotors and gene bodies (≥70% if using human genomic DNA)
• As little as 2 hours hands-on time
• Compatible with low inputs – as little as 10 ng genomic DNA
• Unbiased methylation calling
• Ideal for high-throughput screening

The Zymo-Seq RRBS Library Kit features a simple workflow

Material available to download

Zymo-Seq RRBS Library Kit user manual
Zymo-Seq RRBS Library Kit data sheet