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- Request a TransIT-TKO sample
TransIT-TKO® from Mirus Bio is a broad-spectrum, high efficiency, low toxicity, siRNA and plasmid DNA transfection reagent for mammalian cells. Ideal for a range of hard-to-transfect cells, TransIT-TKO ensures reproducible, targeted gene knockdowns.
Benefits of using TransIT-TKO
• Broad spectrum siRNA and plasmid delivery
• Low cellular toxicity
• High knockdown efficiency
• Reproducible results - consistent, targeted gene knockdowns
Delivery of fluorescently-labelled siRNA using TransIT-TKO Transfection Reagent
HeLa (70% confluence) cells in 12-well plates were transfected with TransIT-TKO Transfection Reagent (3 μl/well) and Label IT® siRNA Tracker Fluorescein-labeled siRNA duplexes (GREEN, 50 nM final concentration in the well). The cells were incubated for 24 hours post-transfection then fixed and counterstained with TO-PRO®-3 (nuclei, BLUE) (Life Technologies) and Alexa Fluor® 546 Phalloidin (actin, RED) (Life Technologies). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope.
High efficiency endogenous knockdown in iCell® cardiomyocytes
TransIT-TKO transfection reagent was used to transfect iCell® Cardiomyocytes (Cellular Dynamics International) plated at a density of 136,500 cells per well of a 12-well plate pre-coated with fibronectin. 7 days post-plating, triplicate wells were transfected with TransIT-TKO (3-5 µl per well) & non-targeting control siRNA or GAPDH targeting siRNA (50nM per well). 72h post-transfection, GAPDH mRNA was measured relative to 18s rRNA mRNA levels using qRT-PCR & scaled to the expression level of non-targeting control siRNA.
Cell lines tested with TransIT-TKO
|
Cell line (source)
|
Endogenous transcript | Knockdown efficiency |
|---|---|---|
|
BNL CL.2 (mouse liver)
|
MAPK1 | 80% |
| MAPK3 | 83% | |
|
HeLa (human cervix)
|
Lamin A/C | 80% |
| GAPDH | 80% | |
|
Hepa1c1c7 (mouse liver)
|
MAPK1 | 80% |
| MAPK3 | 75% | |
| MEK1 | 75% | |
| PTEN | 80% | |
|
HepG2 (human liver)
|
MAPK1 | 80% |
|
NIH 3T3-L1
|
MAPK1 | 70% |
| MAPK3 | 70% | |
|
Secondary human astrocytes
|
Lamin A/C | 80% |
|
Primary mouse hepatocytes
|
ABC A1 | 70% |
| Lamin A/C | 81% |
Free samples
Request a free sample of TransIT-TKO
Material available for download
TransIT-TKO Transfection Reagent protocol
TransIT-TKO Transfection Reagent quick reference protocol
TransIT-TKO Transfection Reagent SDS
Cell types successfully transfected using TransIT-TKO Transfection Reagent
iCell is a registered trademark of Cellular Dynamics
Alexa Fluor is a registered trademark of Life Technologies Corporation
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| TransIT-TKO Transfection Reagent MIR 2154 · Mirus | MIR 2154 Mirus |
0.4 ml |
£407.00
0.4 ml
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|
| TransIT-TKO Transfection Reagent MIR 2150 · Mirus | MIR 2150 Mirus |
1.5 ml |
£652.00
1.5 ml
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|
| TransIT-TKO Transfection Reagent MIR 2155 · Mirus | MIR 2155 Mirus |
5 x 1.5 ml |
£2831.00
5 x 1.5 ml
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|
| TransIT-TKO Transfection Reagent MIR 2156 · Mirus | MIR 2156 Mirus |
10 x 1.5 ml |
£5232.00
10 x 1.5 ml
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|
