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SARS-CoV-2 B.1.351 variant lentivector packaging kit: pPACK-SPIKE

The SARS-CoV-2 pPACK-SPIKE™ packaging kit can be used to produce high titre SARS-CoV-2 Spike pseudotyped lentivirus particles. The mixes incorporate three critical mutations in the Receptor Binding Domain (RBD) of the Spike protein found in the B.1.351 (S. African) variant - K417N, E484 and N501Y - which have been characterised as critical changes in the Spike sequence. This kit helps researchers to understand how mutations in the Spike protein impact SARS-CoV-2 behaviour and is ideal for vaccine and antiviral efficacy studies under BSL2 conditions.

Benefits of using the pPACK-SPIKE lentivector packaging kit
• High titre SARS-CoV-2 Spike pseudotyped lentivirus particles produced
• Ideal for vaccine and antiviral efficacy studies under BSL2 conditions
• Incorporates K417N, E484 and N501Y mutations found in the B.1.351 (S. African) variant
• Based on SBI’s highly cited pPACKH1 Packaging System
• Uses codon-optimised SARS-CoV-2 K417N, E484K, N501Y “S” protein in place of VSV-G envelope protein
• Available for other strains including the original Wuhan-Hu-1 strain, the D614G Spike protein variant and the B.1.153 variant (also known as 501Y.V2)

How it works
pPACK-SPIKE is easy to use — simply co-transfect the pPACK-SPIKE plasmids and your lentivector construct into 293TN producer cells or other lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.

How it works

pPACK-SPIKE pseudovirus particles efficiently infect ACE2 overexpression cells

pPACK-SPIKE pseudovirus particles efficiently infect ACE2 overexpression cells

pPACK-SPIKE packaging mix was used to generate Spike pseudoviral particles carrying a GFP-reporter vector, concentrated using PEG-it Concentration Reagent, and transduced ACE2-overexpressing cells. GFP imaging shows expression of the GFP reporter only in cells overexpressing ACE2, demonstrating the SARS-CoV-2-like behavior of the Spike pseudoviral particles. Data courtesy of Henry David Herce and Michelle Prew from Dana-Farber Cancer Institute and Harvard Medical School.

Material available for download
pPACK-SPIKE™ B.1.351 RBD Mutations manual
pPACK-SPIKE™ SARS-CoV-2 family (Wuhan-Hu-1, D614G and N501Y Spike proteins) manual

Applications
SARS-CoV-2 vaccine and antiviral efficacy studies
SARS-CoV-2 neutralisation assays
SARS-CoV-2 virus - host surface protein interaction studies
SARS-CoV-2 vaccine and therapeutic development

pPACK-SPIKE lentivector packaging kit citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
pPACK-SPIKE SARS-CoV-2 “S” Pseudotype Lentivector Packaging Mix CVD19-500A-1 · System Biosciences
CVD19-500A-1
System Biosciences
10 reactions £630.00
10 reactions
view
pPACK-SPIKE B.1.351 S1 Mutations, SARS-CoV-2 "S" Pseudotype - B.1.351 (S. African) Variant - S1 Mutations (RBD+D614G) - Lentivector Packaging Mix (XL) CVD19-605A-1 · System Biosciences
CVD19-605A-1
System Biosciences
25 reactions £1254.00
25 reactions
view
pPACK-SPIKE B.1.351 S1 Mutations, SARS-CoV-2 "S" Pseudotype - B.1.351 (S. African) Variant - S1 Mutations (RBD+D614G) - Lentivector Packaging Mix CVD19-600A-1 · System Biosciences
CVD19-600A-1
System Biosciences
10 reactions £630.00
10 reactions
view
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit CVD19-589A-KIT · System Biosciences
CVD19-589A-KIT
System Biosciences
1 kit £1060.00
1 kit
view
pPACK-SPIKE B.1.351 RBD Mutations, SARS-CoV-2 "S" Pseudotype - B.1.351 (S. African) Variant - RBD Mutations - Lentivector Packaging Mix (XL) CVD19-585A-1 · System Biosciences
CVD19-585A-1
System Biosciences
25 reactions £1254.00
25 reactions
view
pPACK-SPIKE B.1.351 RBD Mutations, SARS-CoV-2 "S" Pseudotype - B.1.351 (S. African) Variant - RBD Mutations - Lentivector Packaging Mix CVD19-580A-1 · System Biosciences
CVD19-580A-1
System Biosciences
10 reactions £630.00
10 reactions
view
pPACK-SPIKE N501Y SARS-CoV-2 “S” Pseudotype – N501Y Mutant – Lentivector Packaging Mix CVD19-560A-1 · System Biosciences
CVD19-560A-1
System Biosciences
10 reactions £630.00
10 reactions
view
pPACK-SPIKE D614G, SARS-CoV-2 “S” Pseudotype – D614G Mutant – Lentivector Packaging Mix CVD19-530A-1 · System Biosciences
CVD19-530A-1
System Biosciences
10 reactions £630.00
10 reactions
view
HEK293 “N” SARS-CoV-2 Nucleocapsid Overexpression Stable Cell Line CVD19-220A-1 · System Biosciences
CVD19-220A-1
System Biosciences
>2 million cells £3096.00
>2 million cells
view
HEK293 ACE2 Stable Cell Line CVD19-200A-1 · System Biosciences
CVD19-200A-1
System Biosciences
>2 million cells £3096.00
>2 million cells
view
pPACK-BALD Envelope-Free Lentivector Packaging Mix (XL) LV555A-1 · System Biosciences
LV555A-1
System Biosciences
25 reactions £1083.00
25 reactions
view
pPACK-BALD Envelope-Free Lentivector Packaging Mix LV550A-1 · System Biosciences
LV550A-1
System Biosciences
10 reactions £492.00
10 reactions
view
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit CVD19-609A-KIT · System Biosciences
CVD19-609A-KIT
System Biosciences
1 kit £1060.00
1 kit
view