- In this section:
- Quick-DNA/RNA Microprep Plus protocol
- Quick-DNA/RNA Miniprep kit protocol
- Quick-DNA/RNA Miniprep Plus kit protocol
- Quick-DNA/RNA MagBead protocol
- Quick-DNA/RNA Viral Miniprep protocol
- Quick-DNA/RNA-96 Viral protocol
- Quick-DNA/RNA Viral Magbead protocol
- Quick-DNA/RNA Pathogen Miniprep protocol
- Quick-DNA/RNA Pathogen Magbead protocol
- Quick-DNA/RNA FFPE kit protocol
The Quick-DNA/RNA™ kits from Zymo Research rapidly isolate high quality genomic DNA (gDNA) and total RNA from cells, tissue, blood and biological fluids. Both gDNA and a broad range of RNAs are isolated without the use of phenol, with small RNAs and microRNAs ≥17 nt also recovered with no extra steps required.
Benefits of using the Quick-DNA/RNA kits
• Efficient isolation of gDNA and total RNA (including miRNA) from any sample source
• DNA/RNA is ready for all downstream applications including next generation sequencing (NGS)
• DNA/RNA is eluted in as little as 6 µl, suitable for high sensitivity assays
| Name | Applications | Formats available |
|---|---|---|
| Quick-DNA/RNA kits | Quick method for DNA/RNA isolation from small amounts of cells and tissue. | Spin column Magbead |
| Quick-DNA/RNA Plus kits | Universal technology accommodating any sample input including cultured cells, tissue, blood and tough-to-lyse samples. | Spin column Magbead |
| Quick-DNA/RNA Viral kits | Optimised for low viral copy detection for next generation sequencing and RT-qPCR. Compatible with plasma/serum, cell culture media, biological fluids, swabs and faecal samples. | Spin column Plate MagBead |
| Quick-DNA/RNA Pathogen kits | Optimised for pathogen (virus, bacteria, protozoa) detection for NGS and RT-qPCR. Compatible with vectors (mosquitoes, fleas, ticks) and tissues (mammals, birds) samples. | Spin column Magbead |
| Quick-DNA/RNA FFPE kits | Including deparaffinisation solution for simple paraffin removal without xylene and an optimised proteinase K digestion for maximum recovery of high quality, non-crosslinked DNA and DNA-free RNA. Compatible with all downstream applications including PCR and NGS. | Spin column |
The Quick-DNA/RNA plus technology is universal
The Quick-DNA/RNA Plus technology is universal and accommodates any sample input including cultured cells, any tissue, blood and tough-to-lyse samples.
The Quick-DNA/RNA Plus kits enable single-cell detection
HeLa cells were titrated down to a single cell, and DNA and total RNA were extracted using the Quick-DNA/RNA Microprep Plus Kit. Analysis by RT-qPCR shows high linear recovery of DNA & RNA down to the single-cell level (n=2).
The Quick-DNA/RNA Pathogen kit enables inhibitor-free detection in mosquitoes
Hard-to-lyse and inhibitor-rich mosquito vectors were homogenised using ZR BashingBeads™ and purified using Quick-DNA/RNA Pathogen. Ultra-pure, inhibitor-free West Nile Virus nucleic acids (spike-in) were detected by RT-qPCR down to 40 viral copies.
The Quick-DNA/RNA consistently produces high quality DNA and RNA
DNA and RNA isolated using the Quick-DNA/RNA FFPE Kit are high quality and consistently outperforms RNA isolated using a Supplier Q kit (Avg ΔCt = 3.6) as depicted by the RT-PCR amplification curves (n=4).
Material available for download
Quick-DNA/RNA Microprep Plus protocol
Quick-DNA/RNA Miniprep kit protocol
Quick-DNA/RNA Miniprep Plus kit protocol
Quick-DNA/RNA MagBead protocol
Quick-DNA/RNA Viral Miniprep protocol
Quick-DNA/RNA-96 Viral protocol
Quick-DNA/RNA Viral Magbead protocol
Quick-DNA/RNA Viral datatsheet
Quick-DNA/RNA Pathogen Miniprep protocol
Quick-DNA/RNA Pathogen Magbead protocol
Quick-DNA/RNA Pathogen datatsheet
Quick-DNA/RNA FFPE kit protocol
Quick-DNA/RNA FFPE datatsheet
Products
Note: product availability depends on country - see product detail page.
| Details | Cat number & supplier | Size | Price |
| Quick-DNA/RNA Microprep Plus Kit D7005 · Zymo Research | D7005 Zymo Research |
50 preps |
£544.00
50 preps
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|
| Quick DNA/RNA Miniprep Kit D7001 · Zymo Research | D7001 Zymo Research |
50 preps |
£415.00
50 preps
view
|
| Quick DNA/RNA Miniprep Plus Kit D7003 · Zymo Research | D7003 Zymo Research |
50 preps |
£563.00
50 preps
view
|
| Quick-DNA/RNA™ MagBead R2130 · Zymo Research | R2130 Zymo Research |
4 x 96 preps |
£442.00
4 x 96 preps
view
|
| Quick-DNA/RNA™ MagBead R2131 · Zymo Research | R2131 Zymo Research |
4 x 96 preps |
£1499.00
4 x 96 preps
view
|
In this section
Quick-DNA/RNA Microprep Plus protocol- Quick-DNA/RNA Miniprep kit protocol
- Quick-DNA/RNA Miniprep Plus kit protocol
- Quick-DNA/RNA MagBead protocol
- Quick-DNA/RNA Viral Miniprep protocol
- Quick-DNA/RNA-96 Viral protocol
- Quick-DNA/RNA Viral Magbead protocol
- Quick-DNA/RNA Pathogen Miniprep protocol
- Quick-DNA/RNA Pathogen Magbead protocol
- Quick-DNA/RNA FFPE kit protocol