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OneStep PCR Inhibitor Removal kit

The OneStep ™ PCR Inhibitor Removal kits from Zymo Research are ideal for removing contaminants that inhibit downstream enzymatic reactions (e.g. PCR and reverse transcription) from DNA and RNA preparations. Sample clean-up is as simple as applying, spinning and recovering a sample from the column with column matrices specifically designed for the efficient removal of inhibitors including polyphenolic compounds, humic/fulvic acids, tannins and melanin from the most impure DNA and RNA preparations.

Benefits of using the OneStep PCR Inhibitor Removal kit
• High quality DNA or RNA free of enzymatic inhibitors produced
• Inhibitors including polyphenolics, humic/fulvic acids, tannins and melanin efficiently removed
• Fast, one-step procedure
• DNA and RNA produced is ready for downstream applications including PCR, sequencing and RT
• Elute in as little as 50 µl
• Spin-column or 96-well plate format available depending on your requirements

DNA is efficiently amplified by PCR following humic acid removal with the OneStep PCR Inhibitor Removal kit

DNA is efficiently amplified by PCR following humic acid removal with the OneStep PCR Inhibitor Removal Kit

Amplification of a 200 bp product from DNA containing humic acid that was “treated” with the OneStep PCR Inhibitor Removal kit and DNA containing humic acidthat was "non-treated'. PCR amplification was completely inhibited in the case of the “non-treated” sample. Equal amounts of DNA were used for each PCR and equivalent amounts of the reaction were then analysed in a 2.0% (w/v) agarose/TAE/EtBr gel. The ladder is a 100 bp DNA marker (Zymo Research). Hot start PCR was performed using ZymoTaq™ PreMix (Zymo Research).

Free samples
Request a free OneStep PCR Inhibitor Removal kit sample

Material available for download
OneStep PCR Inhibitor Removal Kit protocol
OneStep-96 PCR Inhibitor Removal Kit protocol

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
OneStep™ PCR Inhibitor Removal Kit D6030 · Zymo Research
D6030
Zymo Research
50 preparations £116.00
50 preparations
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OneStep-96™ PCR Inhibitor Removal Kit D6035 · Zymo Research
D6035
Zymo Research
2 x 96 preparations £340.00
2 x 96 preparations
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