The NxGen® Grade T7 RNA polymerase from LGC Biosearch (Lucigen) is in a highly pure form (99% purity) specifically for next-generation applications. The T7 RNA polymerase catalyzes the 5′ to 3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognises the T7 promoter and terminator sequences with high specificity.
Features of the NxGen T7 RNA Polymerase
|Unit concentration||50,000 U/mL|
|SS exonuclease||500 U <0.1% released|
|DS exonuclease||500 U <0.1% released|
|Endonuclease||500 U <10% converted|
|RNAse contamination||500 U = none detected|
|E. coli 16S rDNA contamination||500 U <10 copies|
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C.
Material available for download
NxGen T7 RNA Polymerase datasheet
Note: product availability depends on country - see product detail page.