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NxGen grade phi29 DNA Polymerase

The NxGen® Phi29 DNA polymerase from LGC Biosearch (Lucigen) is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′ to 5′ proofreading exonuclease function. Capable of up to 70,000 insertions per binding event, the NxGen phi29 DNA Polymerase is ideal for generating high levels of DNA whilst conserving sequence integrity.

Features of the NxGen phi29 DNA polymerase

Unit concentration 10,000 U/mL
Purity (SDS-PAGE) >99%
SS Exonuclease Functional
E. coli 16S rDNA contamination 100 U <10 copies
Endonuclease 100 U <10% converted
Source Recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29

Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.

Material available for download
NxGen phi29 DNA Polymerase manual
NxGen phi29 DNA Polymerase SDS


Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
NxGen® phi29 DNA Polymerase 30221-1 · Lucigen
2000 units £183.00
2000 units
NxGen® phi29 DNA Polymerase 30221-2 · Lucigen
10000 units £747.00
10000 units