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Large & difficult to clone fragment cloning kits

Lucigen's large and difficult-to-clone fragment BigEasy® v2.0 linear cloning system is ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb, the linearised pJAZZ vector prevents torsional stress caused by supercoiling, stabilising otherwise unclonable sequences.

Benefits of using the BigEasy® v2.0 linear cloning system
• Maximum insert stability
• Efficiently clone any insert up to 30 kb
• Create libraries from A/T-rich or G/C-rich genomes
• Clone gene clusters or operons
• Inducible copy number

BigEasy-TSA™ electrocompetent cells
The pJAZZ linear cloning vector is maintained at low copy number (5-10/cell) in BigEasy-TSA™ electrocompetent cells (>4 x 1010 cfu/µg), which are required for transformation and propagation. The copy number can be induced 5-10x in this strain.

The BigEasy® long PCR cloning kits contain everything needed to efficiently clone blunt or G-tailed PCR products up to 30 kb into the unbiased, high-fidelity pJAZZ linear cloning vector.
1. Proofreading PCR polymerases (pJAZZ-OK Blunt Vector)
2. Nonproofreading PCR polymerases (pJAZZ-OK GC Vector)

Material available for download
BigEasy® v2.0 linear cloning kits manual
BigEasy-TSA™ electrocompetent cells manual
BigEasy® v2.0 linear cloning kits datasheet

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OC Blunt Vector) 43018-1 · Lucigen
43018-1
Lucigen
5 reactions £430.00
5 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OC Blunt Vector) 43018-2 · Lucigen
43018-2
Lucigen
10 reactions £735.00
10 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OC Blunt Vector) 43018-3 · Lucigen
43018-3
Lucigen
20 reactions £1315.00
20 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OC NotI Vector) 43024-1 · Lucigen
43024-1
Lucigen
5 reactions £430.00
5 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OC NotI Vector) 43024-2 · Lucigen
43024-2
Lucigen
10 reactions £735.00
10 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OK Blunt Vector) 43036-1 · Lucigen
43036-1
Lucigen
5 reactions £430.00
5 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OK Blunt Vector) 43036-2 · Lucigen
43036-2
Lucigen
10 reactions £735.00
10 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OK Blunt Vector) 43036-3 · Lucigen
43036-3
Lucigen
20 reactions £1315.00
20 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OK Notl Vector) 43042-1 · Lucigen
43042-1
Lucigen
5 reactions £430.00
5 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OK Notl Vector) 43042-2 · Lucigen
43042-2
Lucigen
10 reactions £735.00
10 reactions
view
BigEasy® v2.0 Linear Cloning Kit (pJAZZ®-OK Notl Vector) 43042-3 · Lucigen
43042-3
Lucigen
20 reactions £1315.00
20 reactions
view