General Cloning Kits

Cambridge Bioscience offers a range of general use PCR cloning kits from Lucigen.

CloneSmart® Cloning Kits
Cambridge Bioscience offers the CloneSmart® cloning kits, ideal for constructing shotgun libraries, general purpose cloning or cloning blunt PCR products, especially when amounts of target DNA are limited. Containing pre-cut, dephosphorylated pSMART® cloning vector pre-mixed with buffer and ATP, as well as ligase, sequencing primers and DNA controls, this range of convenient kits eliminate hours of tedious preparation, whilst enabling high efficiency cloning.

GC Cloning and Amplification
We also offer the GC cloning kits for the amplification and cloning of PCR products into an unbiased, high-fidelity vector. Containing pre-cut, dephosphorylated pGC Blue cloning vector pre-mixed with buffer and ATP, as well as the CloneSmart® DNA ligase, EconoTaq® DNA Polymerase, sequencing primers, and DNA controls, achieve higher cloning efficiencies and accuracy with resulting GC, rather than TA ends.

E. cloni® Competent Cells
For maximum cloning efficiency, we recommend the use of the E. cloni® 10G ELITE or 10G SUPREME electrocompetent cells. For less demanding applications, E. cloni® chemically competent cells may be used.

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Material Available for Download
CloneSmart® Blunt Cloning Kits Manual
pGC™ Blue Cloning & Amplification Kits Manual