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General cloning kits: CloneSmart

The CloneSmart kits from Lucigen are ideal for constructing shotgun libraries, general purpose cloining or for cloning blunt PCR products, especially with limited target DNA samples. Containing pre-processed vectors and high efficiency competent cells, these kits eliminate hours of tedious preparation and enable efficient cloning.

With no clean-up required, ligation can be completed in as little as 30 minutes with only a brief heat denaturation necessary before transformation, whilst the small vector size (1.7-2.0 kb), facilitates downstream manipulation by mutagenesis or subsequent insert addition. All cloning kits include the transcription- and translation-free pSMART® vectors, which eliminate many of the problems associated with cloning recalcitrant DNA in conventional plasmid vectors.

Benefits of using the CloneSmart kits
• Clone individual targets or libraries with high efficiencies
• Stabilise toxic inserts with optimised vector design
• Choose high or low copy number
• Save time with convenient ligation-ready cloning vectors

High copy and low copy versions of the pSMART transcription-free cloning vectors are available

pSMART vectors

Ori, origin of replication; Amp, ampicillin resistance gene; Kan, Kanamycin resistance gene; ROP, Repressor of primer (lowers plasmid copy number). Approximate positions of sequencing primers and transcription terminators (T) are indicated.

Material available for download
CloneSmart blunt cloning kits manual

CloneSmart citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
CloneSmart HCAmp Blunt Cloning Kit 40041-2 · Lucigen
40041-2
Lucigen
20 reactions £375.00
20 reactions
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CloneSmart LCAmp Blunt Cloning Kit 40300-2 · Lucigen
40300-2
Lucigen
20 reactions £450.00
20 reactions
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CloneSmart HCKan Blunt Cloning Kit 40704-2 · Lucigen
40704-2
Lucigen
20 reactions £379.00
20 reactions
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CloneSmart LCKan Blunt Cloning Kit 40821-2 · Lucigen
40821-2
Lucigen
20 reactions £446.00
20 reactions
view