Exosome transfection system: EV Shuttle Kits

The EV Shuttle Kits from System Biosciences (SBI) provide an easy-to-use, exosome-based system for the delivery of nucleic acids including siRNA, miRNAs, mRNAs and plasmid DNA to a variety of hard-to-transfect cells. Taking advantage of the natural ability of exosomes to be internalised into cells, ready-to-use exosomes derived from human embryonic kidney cells (HEK 293) or murine dendritic cells (JAWS) can be transfected with your nucleic acid of choice, ready for transport and delivery to target cells.

Benefits of the EV Shuttle Kits
• High transfection efficiency
• Simple protocol
• Enables non-viral transduction and stable cell line creation
• Positive control included 
• Provides an alternative gene delivery method 

EV-Entry system
The EV-Entry system enhances the rate of uptake of extracellular vesicles (EVs) by the recipient cells and the offloading of the transported cargo into the cytoplasm of the recipient cells, boosting the delivery of both RNA and protein cargo. The EV-Entry exosome delivery reagent is available separately (cat# EVEN105A-1/EVEN110A-1).

Exosome loading protocol
Simply combine the isolated exosomes provided in the kit with Exo-Fect solution and the nucleic acid of your choice to generate exosome delivery vehicles. The protocol takes less than an hour and is highly efficient at loading nucleic acids into exosomes for transport and delivery.

Human EV shuttles were transfected with Exo-Fect solution and compared to Lipofectamine® 2000 on RAWS 264.7 cells using the exact same amount of Texas-Red labeled siRNA (100 pmol, 10x mag). EV shuttles are more efficient at transfecting siRNA into transfection-resistant cells in culture.

EV Shuttles deliver functional siRNA that can knockdown protein expression in recipient cells

Human HEK293 EV Shuttles were loaded with either an anti-CD81 siRNA or a non-targeting siRNA (NT) and then added to Mouse RAWS 264.7 cells. The cell lysates were collected after 18 hours for Western blot analysis of CD81 protein expression. EV Shuttles carrying the anti-CD81 siRNA were able to reduce CD81 protein expression in the target cells by at least 50% compared to EV Shuttles carrying the NT control siRNA.

Material available for download
EV Shuttle Kits Product information sheet
Human EV Shuttle protocol
Mouse EV Shuttle protocol
EV-Entry System protocol

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