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BAC cloning kits: CopyRight

The CopyRight® v2.0 BAC Cloning Kits from Lucigen provide complete large-insert BAC cloning and library construction projects with unprecedented accuracy, reliability and efficiency when used with the pSMART® BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells. The easy-to-use CopyRight v2.0 kits contain optimised reagents, ligation-ready vector, prepared electrocompetent cells and detailed instructions for high-efficiency cloning.

Benefits of using the CopyRight v2.0 BAC cloning kits
• Efficiently clone any size insert up to 200 kb
• Stabilise toxic or difficult inserts with CloneSmart® technology
• Clone gene clusters or whole operons
• Create libraries from A/T- or G/C-rich genomes
• Induce BAC copy numbers for higher-yield DNA preps

CopyRight vector pSMART BAC

CopyRight vector pSMART BAC. ori2, repE, IncC - single-copy origin of replication; oriV - inducible origin of replication; par A,B,C - partition genes; Cmr - chloramphenicol-resistance gene; cosN - lambda packaging signal; T - CloneSmart transcription terminators; sacB -sucrase gene lacZ - alpha peptide of the beta galactosidase gene. Approximate positions of sequencing primers and transcription terminators are indicated.

Material available for download
CopyRight v2.0 BAC Cloning Kits Manual

CopyRight v2.0 BAC cloning kits citations


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