Reagents for Western blot workflow optimisation

Azure Biosystems offers a range of high-performance reagents to optimise your Western blotting workflow, including the Flash Western kit, Western blotting membranes and washing and blocking buffers.

The Azure Flash Western kit enables researchers to run an entire Western blot from gel to imaging in just 2 hours. Using a proprietary set of reagents, this kit achieves complete membrane blocking, rapid hybridisation of antibodies and enhancement of chemiluminescent signal in less than an hour of blotting time.

Benefits of using the Azure Flash Western kit
• Run entire Western blot in just 2 hours
• Fast protocol with few hands-on steps
• Antibody stabilisation for enhanced chemiluminescent signal
• Low background & increased signal-to-noise ratios
• Increased detection sensitivity & linear dynamic range

Azure Flash Western kit produces more sensitive detection than the traditional protocol with over 90% of antibodies

Azure Flash Western kit produces more sensitive detection than the traditional protocol with over 90% of antibodies

Replicate Western blots were performed using the Azure Flash Western Demo Kit and a traditional Western blot protocol. Each blot shows a dilution series of the same sample. STAT-3 and pSTAT-3 demonstrate the improved data quality possible with the Azure Flash Western Demo Kit, the HSP60 antibody is an example of an antibody with similar performance.

Azure Flash Western kit can reduce a three-day protocol down the <2 hours

Azure Flash Western kit can reduce a three-day protocol down the <2 hours

 

Full workflow optimisationAzure reagents
Azure Biosystems also offer nitrocellulose and PVDF transfer membranes to maximise Western blot success and pre-cut blotting papers to save time and reduce waste for the easiest Western blotting workflow. There are also ready-to-use blocking buffers available, formulated to enhance chemiluminescent or fluorescent signals as well as an all-in-one wash buffer to fully optimise your workflow.

Material available for download
Azure Flash Western chemiluminescence kit brochure
Azure chemiluminescent Western protocol
Flash Western chemiluminescence kit short protocol
Accessories for Western blotting brochure
Application note - chemiluminescent & fluorescent Westerns: choose the best assay for your experiment
Application note – how to improve your chemiluminescent Western blots
Tip sheet: transition from chemiluminescent to fluorescent Western blots

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Flash Western Kit - Rabbit AC2220 · Azure Biosystems
AC2220
Azure Biosystems
1 kit £250.00
1 kit
view
Flash Western Kit-Mouse AC2221 · Azure Biosystems
AC2221
Azure Biosystems
1 kit £250.00
1 kit
view
Nitrocellulose Transfer Membrane 0.45 um AC2106 · Azure Biosystems
AC2106
Azure Biosystems
10 sheets £60.00
10 sheets
view
Nitrocellulose Transfer Membrane 0.22 um AC2107 · Azure Biosystems
AC2107
Azure Biosystems
10 sheets £60.00
10 sheets
view
Low Fluorescence Western Membrane (PVDF) AC2108 · Azure Biosystems
AC2108
Azure Biosystems
10 sheets £230.00
10 sheets
view
Low Fluorescence Western Membrane (PVDF) AC2109 · Azure Biosystems
AC2109
Azure Biosystems
10 sheets £280.00
10 sheets
view
Azure Protein Free Blot Blocking Buffer AC2112 · Azure Biosystems
AC2112
Azure Biosystems
200 ml £150.00
200 ml
view
Azure Fluorescent Blot Washing Buffer AC2113 · Azure Biosystems
AC2113
Azure Biosystems
500 ml £140.00
500 ml
view
Blotting Paper for Western Blotting 7 x 9 cm AC2110 · Azure Biosystems
AC2110
Azure Biosystems
60 sheets £40.00
60 sheets
view
Blotting Paper for Western Blotting 8 x 10 cm AC2111 · Azure Biosystems
AC2111
Azure Biosystems
60 sheets £40.00
60 sheets
view
Azure Chemi Blot Blocking Buffer AC2148 · Azure Biosystems
AC2148
Azure Biosystems
500 ml £150.00
500 ml
view