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High Efficiency Competent Cells for Protein Expression
Lipid-Free RNAi Transfection Reagent

Select-a-Size DCC

Highly effective & efficient in vitro delivery of Cas9 RNP for genome editing in challenging cells

E. cloni® EXPRESS BL21(DE3) competent cells

E. cloni® EXPRESS BL21(DE3) competent cells

These highly efficient electrocompetent cells enable all-inclusive cloning and protein expression. Providing high efficiency cloning and high level protein expression in the same cell, the E. cloni® EXPRESS BL21(DE3) competent cells eliminate the need for plasmid transfer from cloning strain to expression strain and are the ideal solution for routine protein expression applications.

Benefits of using E. cloni EXPRESS BL21(DE3) electrocompetent cells
• Highest efficiency BL21(DE3) cells available - greater than 5 × 10^9 cfu/µg
• High level protein expression
• Eliminate plasmid transfer from cloning strain to expression strain
• Cloning and expression experiments streamlined
• For use with expression plasmids containing the T7 promoter

NOTE: For best results in expressing proteins that may be toxic, we recommend using OverExpress Cells

Transformation efficiency comparison of commercially available BL21(DE3) competent cells

E.cloni EXPRESS Efficiency

Comparison of cloning and protein expression procedures

Workflow

Genotype
Genotype F– ompT hsdSB (rB- mB-) gal dcm lon λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

E. cloni EXPRESS BL21(DE3) strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

E. cloni EXPRESS BL21(DE3) chemically competent cells
We also offer E. cloni EXPRESS chemically competent cells. Offering a high transformation efficiency of over 1 × 10^7 cfu/µg, these chemically competent cells are ideal for everyday protein expression work.

Material available for download
E. cloni® EXPRESS Electrocompetent Cells manual
E. cloni® EXPRESS Chemically Competent Cells manual

Products

Note: availability depends on country - see product detail page.

Contact Our Lucigen Specialist, Doaa