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Pico Methyl-Seq™ Library Prep Kit

Pico Methyl-Seq™ Library Prep Kit

The Pico Methyl-Seq™ Library Prep Kit enables all-inclusive bisulphite conversion and Whole Genome Bisulphite Sequencing (WGBS) library preparation. Providing a streamlined, ligation- and gel-free method and accommodating inputs as low as 10 pg DNA (including that derived from FFPE samples), this kit is ideal for methylation analysis of precious, limited and target-enriched samples.

Benefits of using the Pico Methyl-Seq Library Prep Kit
• All-inclusive kit for bisulphite conversion and WGBS library prep
• Simple, ligation- and gel-free workflow
• Ultra-low input ≥ 10 pg DNA
• FFPE samples compatible
• Whole-genome analysis of 5-methlycytosine enabled

Pico Methyl-Seq offers a simple protocol for WGBS library preparation

Workflow

 

Validation/quantification

Validation of Pico Methyl-Seq libraries. Agilent 2200 TapeStation® D1K gel of libraries prepared (from B1-G1) using 10 pg, 20 pg, 100 pg, 1 ng, 10 ng, and 100 ng, respectively.

Applications
• WGBS Library Preparation
• Epigenetic analysis of DNA methylation

Material available for download
Pico Methyl-Seq™ Library Prep Kit protocol

TapeStation® is a registered trademark of Agilent Technologies, Inc.

Products

Note: availability depends on country - see product detail page.

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