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Episomal DNA Vectors

Cambridge Bioscience offers episomal DNA vectors (Minicircles). Produced as circular expression cassettes devoid of any bacterial plasmid DNA backbone, their smaller molecular size enables more efficient transfections and offers sustained expression over a period of weeks as compared to standard plasmid vectors that only work for a few days.

Benefits of Using the Minicircle DNA Vector Technology
• Ultra-pure minicircle DNA
• Highly reproducible
• Efficient removal of gDNA
• Up to 14 days expression in dividing cells and longer for non-dividing cells
• For use in vivo or in vitro
• Parental plasmid is degraded, preventing immune responses
• Optimised E. coli production strain
• Choice of promoter, reporter gene and vector format

Custom Minicircle DNA Vector Services
We offer, in collaboration with System Biosciences, access to a custom minicircle DNA vector service. To request further information or pricing for this service, please Click Here

Material Available for Download
Minicircle DNA Production System User Manual
Minicircle DNA Vector Technology Datasheet

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Pre-made Minicircle CMV-GFP-SV40PolyA (positive control) MN601MC-1 · System Biosciences
MN601MC-1
System Biosciences
40 µg £610.00
40 µg
view
Pre-made Minicircle CMV-rRFP-SV40PolyA (positive control) MN602MC-1 · System Biosciences
MN602MC-1
System Biosciences
40 µg £610.00
40 µg
view
ZYCY10P3S2T E. coli Minicircle producer strain (competent, ready to transform) MN900A-1 · System Biosciences
MN900A-1
System Biosciences
5 vials POA
5 vials
view
pMC.CMV-GFP-T2A-Puro-H1-Scramble (shRNA scramble control parental minicircle plasmid) MNSI000A-1 · System Biosciences
MNSI000A-1
System Biosciences
10 µg POA
10 µg
view
pMC.CMV-GFP-H1-MCS (shRNA expression parental minicircle plasmid) MNSI500A-1 · System Biosciences
MNSI500A-1
System Biosciences
10 µg POA
10 µg
view
pMC.CMV-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) MNSI505A-1 · System Biosciences
MNSI505A-1
System Biosciences
10 µg POA
10 µg
view
pMC.EF1-GFP-T2A-Puro-H1-MCS (shRNA expression parental minicircle plasmid) MNSI506A-1 · System Biosciences
MNSI506A-1
System Biosciences
10 µg POA
10 µg
view
MC-Easy Minicircle DNA Production Kit (does not include ZYCY10P3S2T producer E. coli strain) MN910A-1 · System Biosciences
MN910A-1
System Biosciences
5 preparations £599.00
5 preparations
view
MC-Easy Minicircle DNA Production Kit (Includes ZYCY10P3S2T producer E. coli strain) MN920A-1 · System Biosciences
MN920A-1
System Biosciences
5 preparations POA
5 preparations
view
Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase DNA (30 ug) BLIV500MC-1 · System Biosciences
BLIV500MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: CMV-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV500MN-1 · System Biosciences
BLIV500MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase DNA (30 ug) BLIV501MC-1 · System Biosciences
BLIV501MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: CMV-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV501MN-1 · System Biosciences
BLIV501MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase DNA (30 ug) BLIV502MC-1 · System Biosciences
BLIV502MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: EF1-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV502MN-1 · System Biosciences
BLIV502MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase DNA (30 ug) BLIV503MC-1 · System Biosciences
BLIV503MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: EF1-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV503MN-1 · System Biosciences
BLIV503MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase DNA (30 ug) BLIV504MC-1 · System Biosciences
BLIV504MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: UBC-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV504MN-1 · System Biosciences
BLIV504MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase DNA (30 ug) BLIV505MC-1 · System Biosciences
BLIV505MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: UBC-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV505MN-1 · System Biosciences
BLIV505MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase DNA (30 ug) BLIV506MC-1 · System Biosciences
BLIV506MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: MSCV-RFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV506MN-1 · System Biosciences
BLIV506MN-1
System Biosciences
10 µg £821.00
10 µg
view
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase DNA (30 ug) BLIV507MC-1 · System Biosciences
BLIV507MC-1
System Biosciences
30 µg £594.00
30 µg
view
Minicircle Dual Reporter: MSCV-GFP-T2A-Luciferase Parental Plasmid (10 ug) BLIV507MN-1 · System Biosciences
BLIV507MN-1
System Biosciences
10 µg £821.00
10 µg
view