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AAV Helper Free Packaging Systems

AAV Helper Free Packaging Systems

Cambridge Bioscience offers adeno-associated virus (AAV) helper-free packaging systems for the efficient packaging of your recombinant adeno-associated virus with your own expression vector without the use of a helper virus. Eliciting very mild immune responses in animal models, the AAV helper-free packaging systems are available for native serotypes 1 through to 6, as well as AAV-DJ and AAV-DJ/8.

AAV Helper-Free System
Most of the adenovirus gene products required for the production of infective AAV particles are supplied on the pHelper plasmid (i.e. E2A, E4, and VA RNA genes) that is co-transfected into cells with human AAV vector DNA. The remaining adenoviral gene product is supplied by the 293 host cells, which stably expresses the adenovirus E1 gene. By eliminating the need for live helper virus, the AAV helper-free system provides a safer and more convenient gene delivery system.

Viral Gene Delivery using the AAV Helper Free System

Available AAV Helper-Free Systems
We offer a range of AAV helper free packaging systems
1. AAV native serotypes 1 - 6
2. AAV-DJ - Engineered by DNA family shuffling to create a hybrid capsid from 8 different native serotypes. AAV-DJ vectors convey significantly higher infectivity rates compared to native serotypes across a broad range of tissues

3. AAV-DJ/8 - AAV-DJ/8 is a mutant of AAV-DJ, which is similar to native serotypes 8 and 9 in its ability to infect heart & brain tissue

AAV Expression Vectors
We offer a range of AAV expression vectors. These vectors are designed to work with any AAV packaging system including our AAV helper-free packaging systems. Find out more Here

AAV helper free expression systems are also available. To find your expression system of interest, speak to our Cell Biolabs Specialist by clicking Here

AAV Control Vectors
We also offer AAV control vectors. These control vectors can be co-transfected along with AAV packaging vectors to produce a recombinant AAV control. Find out more Here

AAV-1 Helper Free Packaging System Citation List

Material Available for Download

AAV-1 Helper Free Packaging System

Note: AAV-DJ Helper Free Systems are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please Contact Us for license information.


Note: availability depends on country - see product detail page.

Contact Our Cell Biolabs Specialist, Doaa