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Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) monoclonal antibody

Catalogue number:
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HA1031
Supplier:
HuaBio
Size:
500 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Applications:

IP, ELISA, IHC, WB

Antibody Host:

Alpaca

Species Reactivity:

Rabbit

Antibody Type:

Alpaca monoclonal Antibody

Immunogen:

Fc region of Rabbit IgG

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Product Description:

Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) monoclonal antibody - 500 µl

Storage Temperature:

Store at 4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Fig2: The Cross-reactions of the seconda
Fig2: The Cross-reactions of the secondary antibody (HA1031) with Mouse IgG and Human IgG.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031) was used for 1 hour at room temperature at 1:80,000 (Lane A, C) and 1:160,000 (Lane B, D).Lane A, B: Mouse IgG (30 ng per lane).Lane C, D: Human IgG (30 ng per lane).Exposure time: 2 minutes
Fig3: Immunohistochemical analysis of pa
Fig3: Immunohistochemical analysis of paraffin-embedded human Colorectal cancer tissue using rabbit anti-Cytokeratin 18 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200 dilution) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.The inset negative control image is secondary-only at 1/200 dilution.
Fig4: Immunohistochemical analysis of pa
Fig4: Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG Fc, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.