siPOOL positive/negative/transgene control 2

siPOOL positive/negative/transgene control 2nmol. neg. control siPOOL has been designed to have no significant sequence similarity to mouse, rat and human sequences. neg. control siPOOL has been tested in multiple cell lines and shows no significant impact on cell proliferation, apoptosis or cell morphology. Pos. control siPOOLs are pre-validated siPOOLs that produce defined characteristics when introduced into cells, indicating successful transfection. Pos. control siPOOLs available include siPOOLs against human PLK1 (5347) and KIF11 (3832) which both produce mitotic arrest and observable cell death; human INCENP (3619) which produces enlarged nuclei observable under the microscope; and human GAPDH (2597) which produces no observable phenotype but exhibits strong decrease in GAPDH RNA levels verifiable by quantitative PCR. Pos. control siPOOLs against mouse Plk1 (18817) and Kif11 (16551) are also available. Transgene siPOOL is a validated siPOOL against EGFP that is used for targeted downregulation of artificially expressed GFP-tagged transcripts.

• MSDS: View or download
• Protocol: View or download