F(ab')2 Cat IgG (H&L) Antibody Alkaline Phosphatase Conjugated

F (ab') 2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F (ab) 2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F (ab) 2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F (ab) 2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F (ab') 2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F (ab') 2 Anti-Cat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Cat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.