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Mouse IgG (H&L) Secondary Antibody Biotin Conjugated

Catalogue number:
610-4602-0100
Size:
100 µg _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£84.00 Estimated delivery Thu 4 Apr
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Applications:

ELISA, Immunohistochemistry, Western Blot

Antibody Host:

Rabbit

Species Reactivity:

Mus musculus (Mouse)

Antibody Isotype:

IgG

Antibody Type:

Polyclonal

Immunogen:

Mouse IgG whole molecule

Alternative Names:

rabbit anti-Mouse IgG Antibody biotin conjugation, rabbit anti-Mouse IgG biotin conjugated Antibody

Product Description:

Anti-Mouse IgG Biotin Antibody generated in rabbit detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them) , activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F (c) region, recognized by high-affinity Fc receptor proteins, as well as the F (ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Mouse secondary antibody conjugated to biotin is available in a variety of formats. Anti-Mouse IgG Biotin Antibody has been tested by ELISA and is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

Storage Temperature:

Store secondary antibody conjugate at 4°C prior to restoration.   For extended storage aliquot conte

ELISA results of purified Rabbit Anti-Mo
ELISA results of purified Rabbit Anti-Mouse IgG Biotin Conjugated Antibody tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (green line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10,000, and TMB-8000 substrate.
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